{"id":2302,"date":"2024-09-29T09:44:07","date_gmt":"2024-09-29T09:44:07","guid":{"rendered":"https:\/\/bestlasercuttingmachine.com\/?post_type=news&p=2302"},"modified":"2024-09-29T09:44:11","modified_gmt":"2024-09-29T09:44:11","slug":"depasser-la-limite-de-diffraction-de-limagerie-chimique-sans-marquage","status":"publish","type":"news","link":"https:\/\/bestlasercuttingmachine.com\/fr\/news\/breaking-the-diffraction-limit-of-label-free-chemical-imaging\/","title":{"rendered":"D\u00e9passer la limite de diffraction de l'imagerie chimique sans marquage"},"content":{"rendered":"

L'imagerie optique \u00e0 super-r\u00e9solution joue un r\u00f4le important dans l'\u00e9tude des m\u00e9canismes microscopiques des processus biologiques. Gr\u00e2ce \u00e0 la technologie d'imagerie \u00e0 super-r\u00e9solution par marquage fluorescent, l'imagerie de macromol\u00e9cules telles que les prot\u00e9ines dans des cellules vivantes a \u00e9t\u00e9 r\u00e9alis\u00e9e avec succ\u00e8s \u00e0 l'\u00e9chelle nanom\u00e9trique. Cependant, le marquage fluorescent n\u00e9cessite une pr\u00e9paration complexe et ne permet pas de marquer efficacement les m\u00e9tabolites de petites mol\u00e9cules (tels que les sucres, les lipides, etc.). La diffusion Raman stimul\u00e9e (SRS) est une technologie d\u2019imagerie sans marquage qui permet d\u2019obtenir une imagerie chimique en capturant les vibrations intrins\u00e8ques des liaisons chimiques des mol\u00e9cules cellulaires. Cependant, la r\u00e9solution spatiale de la SRS n\u2019est que d\u2019environ 300 nanom\u00e8tres, ce qui est insuffisant pour capturer les nanostructures fines pr\u00e9sentes dans les cellules. Par cons\u00e9quent, la question de savoir comment r\u00e9aliser une imagerie chimique sans marquage et \u00e0 super-r\u00e9solution d\u2019\u00e9chantillons biologiques reste un enjeu majeur dans le monde universitaire.<\/p>\n\n\n\n

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R\u00e9cemment, le groupe de Hilton B. de Aguiar, du laboratoire Kastler-Brossel en France, a pr\u00e9sent\u00e9 dans la revue *Advanced Imaging* une technologie de super-r\u00e9solution baptis\u00e9e \u201c Blind-S3 \u201d, qui combine avec succ\u00e8s la SRS et la microscopie \u00e0 illumination structur\u00e9e (SIM). Par rapport \u00e0 la SIM \u00e0 champ large traditionnelle, les auteurs ont con\u00e7u une m\u00e9thode d\u2019imagerie combinant des secondes de balayage \u00e0 champ large et ponctuel, en s\u2019appuyant sur les caract\u00e9ristiques de la SRS. L\u2019\u00e9quipe de recherche a utilis\u00e9 un \u00e9clairage par speckle pour la lumi\u00e8re de pompage (Pump) et a captur\u00e9 des images SRS \u00e0 l\u2019aide d\u2019un seul d\u00e9tecteur en balayant au laser le faisceau de Stokes. Par la suite, une s\u00e9rie d\u2019images d\u2019\u00e9clairage ponctuel a \u00e9t\u00e9 reconstruite \u00e0 l\u2019aide de m\u00e9thodes de calcul avanc\u00e9es. Cette strat\u00e9gie collaborative \u00ab instrument + calcul \u00bb permet non seulement de surmonter le probl\u00e8me li\u00e9 \u00e0 l\u2019impossibilit\u00e9 de d\u00e9tecter les signaux SRS par les cam\u00e9ras, mais aussi d\u2019am\u00e9liorer la profondeur de p\u00e9n\u00e9tration de la SRS gr\u00e2ce \u00e0 l\u2019utilisation de l\u2019\u00e9clairage par speckle. Blind-S3 a permis de doubler la r\u00e9solution et a d\u00e9montr\u00e9 son efficacit\u00e9 en r\u00e9alisant des images de cellules HeLa et de coupes de tissu c\u00e9r\u00e9bral de souris. Cette technologie ne n\u00e9cessite ni irradiation laser de forte puissance ni traitement particulier des \u00e9chantillons, et offre de larges perspectives d\u2019application en cytologie biologique.
Malgr\u00e9 les progr\u00e8s significatifs r\u00e9alis\u00e9s par Blind-S3, sa r\u00e9solution reste encore bien en de\u00e7\u00e0 des capacit\u00e9s de l'imagerie par super-r\u00e9solution \u00e0 fluorescence. Cette limitation n'est pas seulement due \u00e0 la faible r\u00e9solution de l'excitation dans le proche infrarouge, mais r\u00e9sulte \u00e9galement de la sensibilit\u00e9 limit\u00e9e de la SRS. Par cons\u00e9quent, les nouvelles technologies d\u2019imagerie par vibration capables d\u2019atteindre une r\u00e9solution inf\u00e9rieure \u00e0 100 nanom\u00e8tres tout en conservant une sensibilit\u00e9 aux m\u00e9tabolites non fluorescents restent un domaine de recherche tr\u00e8s actif.<\/p>","protected":false},"excerpt":{"rendered":"

Super-resolution optical imaging plays an important role in exploring the microscopic mechanisms of biological processes. Based on super-resolution imaging technology of fluorescent staining, live cell imaging of macromolecules such as proteins has been successfully achieved at the nanoscale. However, fluorescent labeling requires complex preparation and cannot effectively label small molecule metabolites (such as sugars, lipids, […]\n","protected":false},"author":1,"featured_media":2303,"menu_order":0,"comment_status":"open","ping_status":"closed","template":"","tags":[],"news-category":[71],"class_list":["post-2302","news","type-news","status-publish","has-post-thumbnail","hentry","news-category-industry-news"],"yoast_head":"\nBreaking the diffraction limit of label-free chemical imaging - BestLaser<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/bestlasercuttingmachine.com\/fr\/actualites\/depasser-la-limite-de-diffraction-de-limagerie-chimique-sans-marquage\/\" \/>\n<meta property=\"og:locale\" content=\"fr_FR\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Breaking the diffraction limit of label-free chemical imaging - BestLaser\" \/>\n<meta property=\"og:description\" content=\"Super-resolution optical imaging plays an important role in exploring the microscopic mechanisms of biological processes. 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