{"id":2302,"date":"2024-09-29T09:44:07","date_gmt":"2024-09-29T09:44:07","guid":{"rendered":"https:\/\/bestlasercuttingmachine.com\/?post_type=news&p=2302"},"modified":"2024-09-29T09:44:11","modified_gmt":"2024-09-29T09:44:11","slug":"superar-el-limite-de-difraccion-en-la-obtencion-de-imagenes-quimicas-sin-marcadores","status":"publish","type":"news","link":"https:\/\/bestlasercuttingmachine.com\/es\/news\/breaking-the-diffraction-limit-of-label-free-chemical-imaging\/","title":{"rendered":"Superando el l\u00edmite de difracci\u00f3n en la obtenci\u00f3n de im\u00e1genes qu\u00edmicas sin marcadores"},"content":{"rendered":"

La imagenolog\u00eda \u00f3ptica de superresoluci\u00f3n desempe\u00f1a un papel importante en el estudio de los mecanismos microsc\u00f3picos de los procesos biol\u00f3gicos. Gracias a la tecnolog\u00eda de imagenolog\u00eda de superresoluci\u00f3n basada en la tinci\u00f3n fluorescente, se ha logrado con \u00e9xito la obtenci\u00f3n de im\u00e1genes de macromol\u00e9culas, como las prote\u00ednas, en c\u00e9lulas vivas a escala nanom\u00e9trica. Sin embargo, el marcaje fluorescente requiere una preparaci\u00f3n compleja y no permite marcar de forma eficaz metabolitos de mol\u00e9culas peque\u00f1as (como az\u00facares, l\u00edpidos, etc.). La dispersi\u00f3n Raman estimulada (SRS) es una tecnolog\u00eda de imagen sin marcadores que permite obtener im\u00e1genes qu\u00edmicas mediante la captura de las vibraciones intr\u00ednsecas de los enlaces qu\u00edmicos de las mol\u00e9culas celulares. No obstante, la resoluci\u00f3n espacial de la SRS es de solo unos 300 nan\u00f3metros, lo que no resulta suficiente para captar las nanoestructuras m\u00e1s finas de las c\u00e9lulas. Por lo tanto, c\u00f3mo lograr una obtenci\u00f3n de im\u00e1genes qu\u00edmicas de superresoluci\u00f3n sin marcadores de muestras biol\u00f3gicas sigue siendo un tema de gran importancia en el \u00e1mbito acad\u00e9mico.<\/p>\n\n\n\n

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Recientemente, el grupo de Hilton B. de Aguiar, del Laboratorio Kastler-Brossel de Francia, public\u00f3 en la revista *Advanced Imaging* un art\u00edculo sobre una tecnolog\u00eda de superresoluci\u00f3n denominada \u201cBlind-S3\u201d, que combinaba con \u00e9xito la SRS con la microscop\u00eda de iluminaci\u00f3n estructurada (SIM). En comparaci\u00f3n con la SIM de campo amplio tradicional, los autores dise\u00f1aron un m\u00e9todo de obtenci\u00f3n de im\u00e1genes que combina el campo amplio y el escaneo puntual bas\u00e1ndose en las caracter\u00edsticas de la SRS. El equipo de investigaci\u00f3n utiliz\u00f3 iluminaci\u00f3n de moteado para la luz de bombeo (Pump) y captur\u00f3 im\u00e1genes de SRS con un \u00fanico detector mediante el escaneo l\u00e1ser del haz de Stokes. Posteriormente, se reconstruy\u00f3 una serie de im\u00e1genes de iluminaci\u00f3n puntual mediante m\u00e9todos computacionales avanzados. Esta estrategia colaborativa de \u00abinstrumento + c\u00e1lculo\u00bb no solo supera el problema de que las se\u00f1ales de SRS no pueden ser detectadas por las c\u00e1maras, sino que tambi\u00e9n mejora la profundidad de penetraci\u00f3n de la SRS mediante el uso de la iluminaci\u00f3n de moteado. Blind-S3 logr\u00f3 duplicar la resoluci\u00f3n y demostr\u00f3 su eficacia mediante la obtenci\u00f3n de im\u00e1genes de c\u00e9lulas HeLa y de cortes de tejido cerebral de rat\u00f3n. Esta tecnolog\u00eda no requiere irradiaci\u00f3n l\u00e1ser de alta potencia ni un procesamiento especial de las muestras, y presenta amplias perspectivas de aplicaci\u00f3n en citolog\u00eda biol\u00f3gica.
A pesar de los importantes avances logrados por Blind-S3, su resoluci\u00f3n sigue estando muy por debajo de las capacidades de la imagenolog\u00eda de superresoluci\u00f3n por fluorescencia. Esto no solo se debe a la baja resoluci\u00f3n de la excitaci\u00f3n en el infrarrojo cercano, sino que tambi\u00e9n se deriva de la limitada sensibilidad de la SRS. Por lo tanto, las nuevas tecnolog\u00edas de imagen por vibraci\u00f3n capaces de alcanzar una resoluci\u00f3n inferior a 100 nan\u00f3metros, al tiempo que mantienen la sensibilidad frente a los metabolitos no fluorescentes, siguen siendo un \u00e1rea de investigaci\u00f3n de gran inter\u00e9s.<\/p>","protected":false},"excerpt":{"rendered":"

Super-resolution optical imaging plays an important role in exploring the microscopic mechanisms of biological processes. Based on super-resolution imaging technology of fluorescent staining, live cell imaging of macromolecules such as proteins has been successfully achieved at the nanoscale. However, fluorescent labeling requires complex preparation and cannot effectively label small molecule metabolites (such as sugars, lipids, […]\n","protected":false},"author":1,"featured_media":2303,"menu_order":0,"comment_status":"open","ping_status":"closed","template":"","tags":[],"news-category":[71],"class_list":["post-2302","news","type-news","status-publish","has-post-thumbnail","hentry","news-category-industry-news"],"yoast_head":"\nBreaking the diffraction limit of label-free chemical imaging - BestLaser<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/bestlasercuttingmachine.com\/es\/noticias\/superar-el-limite-de-difraccion-en-la-obtencion-de-imagenes-quimicas-sin-marcadores\/\" \/>\n<meta property=\"og:locale\" content=\"es_ES\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Breaking the diffraction limit of label-free chemical imaging - BestLaser\" \/>\n<meta property=\"og:description\" content=\"Super-resolution optical imaging plays an important role in exploring the microscopic mechanisms of biological processes. 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